(A) PM (Lanes 1–9) and (B) PM (Lanes 10–13) and PF (Lanes 14–17) by agarose electrophoresis. Lanes: M, EZ load 100 bp Molecular Ruler (Bio-rad, Hercules, CA); 1, Madurella mycetomatis IFM 46458 (10 ng); 2, M. pseudomycetomatis IFM 46460 (10 ng); 3, M. tropicana CBS 201.38 (10 ng); 4, M. fahalii CBS 129176 (10 ng); 5, Chaetomium rectangulare CBS 126778 (10 ng); 6, M. mycetomatis IFM 46458 (1 pg); 7, M. pseudomycetomatis IFM 46460 (1 pg); 8, M. tropicana CBS 201.38 (1 pg); 9, no template; 10, M. mycetomatis IFM 46458 (10 ng); 11, M. mycetomatis IFM 46458 (1 pg); 12, M. fahalii CBS 129176 (10 ng); 13, no template; 14, M. fahalii CBS 129176 (10 ng); 15, M. fahalii CBS 129176 (1 pg); 16, M. mycetomatis IFM 46458 (10 ng); 17, no template. (TIF)</p