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Verification of LAMP amplicons using primer sets PM, PM and PF by agarose electrophoresis.

Abstract

(A) PM (Lanes 1–9) and (B) PM (Lanes 10–13) and PF (Lanes 14–17) by agarose electrophoresis. Lanes: M, EZ load 100 bp Molecular Ruler (Bio-rad, Hercules, CA); 1, Madurella mycetomatis IFM 46458 (10 ng); 2, M. pseudomycetomatis IFM 46460 (10 ng); 3, M. tropicana CBS 201.38 (10 ng); 4, M. fahalii CBS 129176 (10 ng); 5, Chaetomium rectangulare CBS 126778 (10 ng); 6, M. mycetomatis IFM 46458 (1 pg); 7, M. pseudomycetomatis IFM 46460 (1 pg); 8, M. tropicana CBS 201.38 (1 pg); 9, no template; 10, M. mycetomatis IFM 46458 (10 ng); 11, M. mycetomatis IFM 46458 (1 pg); 12, M. fahalii CBS 129176 (10 ng); 13, no template; 14, M. fahalii CBS 129176 (10 ng); 15, M. fahalii CBS 129176 (1 pg); 16, M. mycetomatis IFM 46458 (10 ng); 17, no template. (TIF)</p

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