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The primers’ design targeting all four Madurella spp. (primer PV2).

Abstract

(A) The alignment of rDNA sequences among Madurella strains and Chaeromium rectangluare (negative control) is shown. Arrows illustrate the primers’ positions. (B) The specificity of primer PV2. LAMP reaction was performed using genomic DNAs derived from Madurella spp. and C. rectangulare at 65°C. Abbreviations: MM, M. mycetomatis; MP, M. pseudomycetomatis; MT, M. tropicana; MF, M. fahalii; CR, Chaetomium rectangluare; B, Blank sample with a buffer used for DNA dilution. (TIF)</p

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