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unknown
Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells
Authors
F. Atyabi
M. Dinarvand
+5 more
A. Ghaemi
M. Kelishadi
A. Moradi
A. Tabarraei
A. Tahamtan
Publication date
1 January 2015
Publisher
Taylor and Francis Ltd.
Abstract
Chitosan nanoparticles (CS NPs) were prepared as a carrier for Human papillomavirus type 16 HPV-16) E7 gene and their gene transfection ability were evaluated in vitro . The plasmid expressing green fl uorescent protein (pEGFP) was used as a reporter gene. Gel electrophoresis demonstrated full binding of CS NPs with the pDNA. The transfection of CS-pEGFP NPs was effi cient in CHO cells and the expression of green fl uorescent proteins was well observed. The expression of E7 proteins was confi rmed under SDS-PAGE and western blot analysis. As a conclusion CS NPs may serve as an eff ective nonviral carrier for delivery of nucleotides into eukaryotic cells. Copyright © 2014 Informa Healthcare USA, Inc
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oai:eprints.goums.ac.ir:4788
Last time updated on 11/11/2016