Smn deficiency induces p53 accumulation but not serine 18 phosphorylation in L1 motor neurons of <i>Smn</i>^<i>2B/-</i> SMA mice.

Abstract

(A) ChAT and p53 immunostaining of the L1 spinal cord from control (Smn2B/+) and SMA (Smn2B/-) mice at P16. Scale bars: 100 μm. (B) ChAT and p53 immunostaining of L1 motor neurons from the same groups as in (A). Scale bars: 50 μm. (C) ChAT and phospho-p53S18 immunostaining of L1 motor neurons from the same groups as in (A). Scale bars: 50 μm. (D) Percentage of p53+ L1 motor neurons from the same groups as in (A). (E) Percentage of phospho-p53S18+ L1 motor neurons from the same groups as in (A). Data represents individual values, mean and SEM from 3 mice per group. Statistics were performed with two-tailed unpaired Student’s t-test. *** P < 0.001.</p