Longitudinal cryosections of immunolabeled aquaporin-4 and phalloidin on human (A-E), and porcine (B) optic nerve head tissue.

Abstract

Minimal label for AQP4 (green) is visible in the lamina cribrosa (between dotted white lines in A, B; seen at higher power in C and E. AQP4 label is prominent in the retinal nerve fiber layer, prelaminar area and myelinated optic nerve in each species. Phalloidin labeling of F-actin (red) is prominent in the prelamina, lamina and myelinated ON (D, E). AQP4 labeling is visible within axon bundles and pronounced along the edge of axons bundles and at the pia surface. DAPI (blue) identifies cell nuclei. Scale Bar: 200 μm (A, D), 50 μm (B, C, E).</p