In vivo luciferase transgene expression by Ad5 and Ad26 ± HD5 pretreatment.

Abstract

Animals were pretreated with a dose titration of 2.5 μM– 100 μM HD5, 100 μM mutant HD5 (linear peptide), or PBS ± 100 μM HD5 and immunized i.m. with (A) Ad5 (109 vp) or (B) 25 μM– 100 μM HD5 with Ad26 (109 vp) vectors encoding luciferase transgene cassettes. Representative day 3 IVIS images are shown for each experimental condition assayed. The Total Flux (photons/sec/cm2/radian) released by luciferase activity was averaged for (C) Ad5 and (D) Ad26 at each time-point. n = 5 animals per group. The data are representative of experiments performed two times. Mean ± SEM are shown. *** p p p < 0.05, one-way ANOVA test (compared to virus only control).</p