Change in plasmid copy numbers 28 days after removal of pyrimethamine.

Abstract

Plasmid copy numbers were quantitated before (day 0) and after (day 28) removal of pyrimethamine drug pressure in transgenic parasites with PvCEN11S2-H86HD-TG (PvCEN11S2), PvCEN11S3-H86HD-TG (PvCEN11S3), PyCEN5-H86HD-TG (PyCEN5), or pNoCEN. Three or five mice were used for each group on days 0 and 28, respectively. After the one-way ANOVA test indicated significant difference, Tukey's post-hoc multiple comparisons test was performed. Significant difference was indicated with p values. n.s. indicates not significant (p ≥ 0.05).</p