<i>P</i>. <i>vivax</i> promoter activities in <i>P</i>. <i>yoelii</i>.

Abstract

Firefly luciferase was expressed under a panel of P. vivax promoters. P. falciparum heat shock protein 86 promoter was used as a positive control, because this promoter has been shown to be active in P. yoelii. A plasmid without a promoter sequence upstream of the luciferase open reading frame was used as a negative control. Luciferase activities from purified schizont stage parasites were normalized by the plasmid copy number of each parasite quantitated by qPCR. Results from two independent experiments are shown. Mean and standard deviation from triplicate wells are plotted.</p