There were four reactions tested: 1, the reference strain of K. pneumoniae ATCC2146 (10 pg per reaction); 2, Staphylococcus aureus (67.5 ng per reaction); 3, Salmonella typhii (130.0 ng per reaction); 4, distilled water. Only the reaction with K. pneumoniae ATCC2146 showed the positive results: blue in color (Fig 2A), ladder bands by gel electrophoresis (Fig 2B), and the two red lines (both the positive line and the control line) indicated on the LFB (Fig 2C).</p