MEF cells lacking BNIP3 show higher levels of MAPK activation compared to MEF cells expressing BNIP3.

Abstract

(A) Total lysates from wild-type and BNIP3 knockout MEFs were western blotted for phospho-MAPK. The blot was stripped and reprobed with total MAPK and GAPDH as loading controls. (B) The protein levels of p-MAPK and total MAPK were measured using ImageJ software. The ratio of p-MAPK/total MAPK was plotted to compare the activation of MAPK. The results are an average of three replicates. The error bars show standard error of the mean. Tukey’s post hoc test was used to calculate statistical significance (*p<0.05). Results shown are representative of three independent experiments Total lysates from wild-type and BNIP3 knockout MEFs were western blotted for Cyclin D1. The blot was stripped and reprobed with GAPDH as loading control. The protein levels were quantified using ImageJ software. The ratio of Cyclin D1 to GAPDH was calculated and normalized to the highest level.</p