(A) Phasor plot depicting the phasor position of control and serum starved cells in the second quadrant of first harmonic. Four cone shaped cursors were utilised to select four distinct λmax values irrespective of the spectral width. (B) Spectral images were produced by remapping the selected emissions in the (A) phasor plot to the original fluorescence images. Notably, the 532, 536, 540 and 544 nm emissions were apparent in both the nucleus and cytoplasm of cells throughout the first 40-minutes post serum starvation.</p