Differential modulation of γD-crystallin protein aggregation by transgenes of murine <i>Cryaa</i> mutants.

Abstract

(A) Schematic of the experiment utilizing the Gal4/UAS targeted gene expression system. Double transgenic line, Tg[cryaa:Gal4]; Tg[UAS:GFP], were outcrossed to either αA-R49C, cryaa-/-;αA-R49C, αA-R116C (shown in Fig 4B) or αB-R120G (shown in Fig 5B) transgenic lines. Fertilized zygotes were injected with tol2 mRNA and UAS responder Tol2 constructs expressing fluorescently tagged human γD-crystallin (Hsa.CRYGD_I4F-mCherry). Embryos possessing lens fiber cells positive for mCherry mosaic expression were selected and imaged at 4dpf. (B) The percentage of embryos showing γD-crystallin_I4F-mCherry punctuates in the lens were significantly increased when Tg(cryaa:Rno.Cryaa_R49C) was co-expressed, but not with Tg(cryaa:Rno.Cryaa_R116C). Error bars represented standard deviations in αA-R49C crosses (left panel,) and standard errors in αA-R116C crosses (right panel). Number of crosses is denoted in parentheses.</p