Hoffman, Kathleen

Kang, Hye-won

Robinson, Phyllis

VCU Scholars Compass

Virginia Commonwealth UniversityVCU Scholars CompassBiology and Medicine Through MathematicsConference 2016May 20th, 11:15 AM - 11:45 AMDeterministic and Stochastic MathematicalModeling of Melanopsin's Light Response iniPRGCs and HEK CellKathleen HoffmanUMBC, khoffman@umbc.eduHye-won KangUMBC, hwkang@umbc.eduPhyllis RobinsonUMBC, probinso@umbc.eduFollow this and additional works at: http://scholarscompass.vcu.edu/bammThis Event is brought to you for free and open access by the Dept. of Mathematics and Applied Mathematics at VCU Scholars Compass. It has beenaccepted for inclusion in Biology and Medicine Through Mathematics Conference by an authorized administrator of VCU Scholars Compass. Formore information, please contact libcompass@vcu.edu.http://scholarscompass.vcu.edu/bamm/2016/May20/1DETERMINISTIC AND STOCHASTIC MATHEMATICAL MODELING OF MELANOPSIN’s LIGHT RESPONSE IN iPRGCS AND HEK CELL   Kathleen Hoffman, Phyllis Robinson, Hye-won Kang  Melanopsin, a visual pigment expressed in intrinsically photosensitive retinal ganglion cells (ipRGCs), primarily affects non-image forming vision with a hypothesized G-protein phototransduction pathway similar to the phototransduction pathway found in Drosophila photoreceptors. The biochemical cascade underlying the light response in ipRGCs has not yet been fully elucidated. A deterministic and stochastic mathematical model of the hypothesized pathway is developed to probe key elements of the pathway. The rate constants, initial conditions and parameters are fit to voltage clamp data from ipRGCs and also calcium imaging data from HEK cells transfected with mouse melanopsin. Sensitivity analysis reveals key steps in the phototransduction pathway, independent of the experimental conditions, as well as key differences attributable to the different experimental conditions.  Acknowledgement: Research has been funded by a grant from NSF for Interdisciplinary Training for Undergraduates in Biological and Mathematical Sciences  (NSF-UBM:1031420) and NSF (IOSO721608) and NEI (R01Y019053) to P.R.R. This is joint work with UBM students Kimberly Daniels, Jaquelyn Meisel, Kevin Herod, Drew Thatcher, Jessica Ortega, Abigail Jackson and Adam Byerly. Stochastic model was developed with the WhAM! Group sponsored by the IMA. We thank Devyani Ujla, Preethi Somasundaram, and Evan G. Cameron calcium imaging data. Dr. R. Lane Brown for provided the electrophysiological data.  

Deterministic and Stochastic Mathematical Modeling of Melanopsin\u27s Light Response in iPRGCs and HEK Cell

http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=1026&context=bamm

Deterministic and Stochastic Mathematical Modeling of Melanopsin\u27s Light Response in iPRGCs and HEK Cell

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