PZQ enantiomers do not activate heterologously expressed TRPV1.

Abstract

(A) Representative fluorescence traces from HEK293 cells loaded with Fluo-4 AM following the addition of capsaicin (1μM, black) or ±PZQ (100μM, purple), followed by addition of ATP (100μM). (B) Representative fluorescence traces from HEK293 cells transfected with hTRPV1 and loaded with Fluo-4 AM following the addition of capsaicin (1μM), followed by addition of ATP (100μM), in the presence of DMSO (0.1%, black) or capsazepine (10μM, brown). (C) Representative fluorescence traces of HEK293 cells transfected with hTRPV1 and loaded with Fluo-4 AM following the addition of ±PZQ (100μM), followed by ATP (100μM), in the presence of DMSO (0.1%, black), or capsazepine (10μM, brown). (D) Cumulative measurements of peak fluorescence ratio (F/F0, where ‘F’ represents fluorescence at peak and ‘F0’ represents fluorescence at time = 0) from Ca2+ imaging experiments under indicated conditions. Data represent population means±s.e.m. (>20 cells) from n≥3 independent transfections.</p