Cockburn, Ian

Ganusov, Vitaly V

Kelemen, Reka

VCU Scholars Compass

Virginia Commonwealth University
VCU Scholars Compass
Biology and Medicine Through Mathematics
Conference 2016
May 21st, 10:45 AM - 11:15 AM
Needle in the haystack: combining intravital
imaging and mathematical modeling to understand
how vaccine-induced T cells find malaria-infected
cells in murine livers
Vitaly V. Ganusov
University of Tennessee, vitaly.ganusov@gmail.com
Ian Cockburn
Australian National University, ian.cockburn@anu.edu.au
Reka Kelemen
Vienna Graduate School, rkelem@gmail.com
Follow this and additional works at: http://scholarscompass.vcu.edu/bamm
Part of the Immunology of Infectious Disease Commons, Medical Immunology Commons, and
the Pathogenic Microbiology Commons
This Event is brought to you for free and open access by the Dept. of Mathematics and Applied Mathematics at VCU Scholars Compass. It has been
accepted for inclusion in Biology and Medicine Through Mathematics Conference by an authorized administrator of VCU Scholars Compass. For
more information, please contact libcompass@vcu.edu.
http://scholarscompass.vcu.edu/bamm/2016/May21/64
After Plasmodium-infected mosquito transmits the malaria parasite to the mammalian host, 
parasites (called sporozoites) travel to the liver and infect hepatocytes.  Within several days, 
sporozoites replicate and differentiate into merozoites which are then able to re-enter circulation 
and infect red blood cells. Removal of sporozoites while they travel to the liver or during their 
replication in the liver prevents clinical symptoms of malaria, and this is why sporozoites have 
been considered as a good target for malaria vaccines. Using intravital imaging we recently 
discovered that vaccine-induced CD8 T cells form clusters around sporozoite-infected 
hepatocytes in murine livers, and that these clusters are associated with parasite removal. Here I 
extend our previous studies and demonstrate that formation of these clusters cannot be driven 
solely by differences in parasite's attractiveness for T cells, while the model in which T cells 
attract other T cells to the site of infection is best at describing our data. Additional analyses also 
suggest that formation of such clusters occurs rather rapidly after initial recognition of the 
infected cell. 
 
 


English

Needle in the haystack: combining intravital imaging and mathematical modeling to understand how vaccine-induced T cells find malaria-infected cells in murine livers

Virginia Commonwealth UniversityVCU Scholars CompassBiology and Medicine Through MathematicsConference 2016May 21st, 10:45 AM - 11:15 AMNeedle in the haystack: combining intravitalimaging and mathematical modeling to understandhow vaccine-induced T cells find malaria-infectedcells in murine liversVitaly V. GanusovUniversity of Tennessee, vitaly.ganusov@gmail.comIan CockburnAustralian National University, ian.cockburn@anu.edu.auReka KelemenVienna Graduate School, rkelem@gmail.comFollow this and additional works at: http://scholarscompass.vcu.edu/bammPart of the Immunology of Infectious Disease Commons, Medical Immunology Commons, andthe Pathogenic Microbiology CommonsThis Event is brought to you for free and open access by the Dept. of Mathematics and Applied Mathematics at VCU Scholars Compass. It has beenaccepted for inclusion in Biology and Medicine Through Mathematics Conference by an authorized administrator of VCU Scholars Compass. Formore information, please contact libcompass@vcu.edu.http://scholarscompass.vcu.edu/bamm/2016/May21/64After Plasmodium-infected mosquito transmits the malaria parasite to the mammalian host, parasites (called sporozoites) travel to the liver and infect hepatocytes.  Within several days, sporozoites replicate and differentiate into merozoites which are then able to re-enter circulation and infect red blood cells. Removal of sporozoites while they travel to the liver or during their replication in the liver prevents clinical symptoms of malaria, and this is why sporozoites have been considered as a good target for malaria vaccines. Using intravital imaging we recently discovered that vaccine-induced CD8 T cells form clusters around sporozoite-infected hepatocytes in murine livers, and that these clusters are associated with parasite removal. Here I extend our previous studies and demonstrate that formation of these clusters cannot be driven solely by differences in parasite's attractiveness for T cells, while the model in which T cells attract other T cells to the site of infection is best at describing our data. Additional analyses also suggest that formation of such clusters occurs rather rapidly after initial recognition of the infected cell.   

https://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=1013&amp;context=bamm

Needle in the haystack: combining intravital imaging and mathematical modeling to understand how vaccine-induced T cells find malaria-infected cells in murine livers

Abstract

Similar works

Full text

Available Versions

VCU Scholars Compass

VCU Scholars Compass