Reciprocal melting temperature versus ln (C/4) for 5′ACUCCA/5′UGGAGU (solid squares), 5′ACUACCA/5′UGGUAGU (solid circles), 5′ACUCCA/5′UGGUAGU (solid stars) and 5′AUCA/5′UGGUAGU (solid triangles) in 100 mM NaCl, 20 mM sodium cacodylate, 0

Abstract

<p><b>Copyright information:</b></p><p>Taken from "The influence of locked nucleic acid residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes"</p><p>Nucleic Acids Research 2005;33(16):5082-5093.</p><p>Published online 9 Sep 2005</p><p>PMCID:PMC1201327.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p>5 mM NaEDTA, pH 7.0. The thermodynamics of duplex formation were obtained by fitting the data points to the equation, = (R/Δ°) ln (C/4) + Δ°/Δ°. Here, is the inverse melting temperature in kelvin, R is the gas constant, 1.987 cal K mol, C is the total oligonucleotide strand concentration, and both strands have the same concentration