Gene profile analysis of osteoblast genes differentially regulated by histone deacetylase inhibitors-3

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Gene profile analysis of osteoblast genes differentially regulated by histone deacetylase inhibitors"</p><p>http://www.biomedcentral.com/1471-2164/8/362</p><p>BMC Genomics 2007;8():362-362.</p><p>Published online 9 Oct 2007</p><p>PMCID:PMC2147034.</p><p></p>ing the indicated HDI or DMSO. mRNAs were isolated at various times over a 6 or 18 hour period and subjected to quantitative real-time PCR with primers for Slc9a3r1 (A and B) or Sdh1 (C and D). Values are relative to those obtained from DMSO-treated samples at each time point and represent the mean of three samples. For (A-D), * denotes a statistically significant change of p < 0.01 and ** denotes p < 0.05 by one-way ANOVA of the HDI-treated sample versus the DMSO-treated sample at that time point. (E) C2C12 cells were cultured in osteogenic medium and 20 nM TSA for the indicated times. Slc9a3r1 protein levels were determined by immunoblotting