Critical role of NF-kB in NCTD-induced transcriptional inhibition of MMP-9 and u-PA in Huh7 cells.

Abstract

<p>Huh7 cells were treated with NCTD 10 µM for 24 h and then the nuclear fraction was prepared as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031055#s4" target="_blank"><i>Materials and Methods</i></a>”. (A and B) ChIP analysis of the association of various transcription factors with the MMP-9 and u-PA promoter region in Huh7 cells. (C and D) Representative results of NF-KB protein levels and phosphorylation of IkBα by Western blot analysis. Quantitative results of NF-kB protein levels, which were adjusted with the internal control Fibrillian or GAPDH protein level. The values represented the means ± SD of at least three independent experiments. <i>*P</i><0.05 as compared with the vehicle group.</p