Binding affinities for 5 MarA variants to 64 binding sites.

Abstract

<p>The heat map shows the relative binding affinities of wild type MarA (WQR) and 4 selected variants to 64 variations of the <i>mar</i> binding site (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002614#pgen-1002614-g001" target="_blank">Figure 1</a>). Each MarA variant (y-axis) is named according to its residues at positions 42, 45 and 46. Each DNA sequence (x-axis) was substituted for ‘NNN’ in the <i>mar</i> binding site (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002614#pgen-1002614-g001" target="_blank">Figure 1</a>). Data for all variants were normalized so the highest affinity site was set to 1 (black). All other sites are colored relative to that site according to the color scale. All sites below 0.3 were colored the same as 0.3.</p