Percentages of Overall and Laboratory Variability by Component.

Abstract

<p>(A) Overall variability in the laboratory process; PCR process  = 70% (speckled white); reagent lots, operators, or machines  = 24% total (grey); unaccounted = 6%. (B) Contributions of laboratory processes to non-PCR associated variability; RNA extraction (white), cDNA (light grey), qRT-PCR (mid grey), sample addition (dark grey), unexplained (black lines).</p