The immediate effect of PR3 or elastase on PAR signaling induced by other stimuli.

Abstract

<p>Fig. 4A–C show the effect of a 10 min pre-treatment of GEC with PR3 (34.5 nM = 1 µg/ml PR3(1) or 172 nM = 5 µg/ml PR3(5)) or elastase (33.9 nM = 1 µg/ml HNE(1), or 169.5 nM = 5 µg/ml HNE(5)), on subsequent PAR1 calcium signaling induced by either thrombin (Fig. 4A) or PAR1ap (Fig. 4B) and on subsequent trypsin activation of PAR2 (Fig. 4C). Baseline calcium levels in untreated cells were recorded (control). The bars marked ‘HBH’ show responses after addition of buffer alone (Hanks balanced salt solution +20 mM HEPES) in the absence of any protease during a 10 min pre-treatment period, followed by stimulation of the cells with either thrombin (Fig. 4A) or PAR1ap (Fig. 4B) or PAR2 (Fig. 4C) were used as positive controls for each experiment. Fig. 4A–C show data from 3–4 independent experiments (mean ± SEM).</p