PPARβ/δ knock-down and antagonist prevent VEGF induction.

Abstract

<p>(<b>A</b>) H358 and H441 cells were transfected with PPARβ/δ siRNA and control GL3 and then treated with GW501516 (5 µM) for 18 h. Gene levels were determined by RT-PCR. (<b>B</b>) Cells were incubated for 2 h with GSK0660 (10 µM) and then with GW501516 (5 µM) for 18 h prior analysis by RT-PCR. (<b>C</b>) H358 cells were treated with GW501516 for 18 h and processed for chromatin immunoprecipitation using anti-PPARβ/δ and anti-IgG antibodies. The region of the VEGF promoter containing a PPRE (−527/−298) and a non-targeted region (−1338/−1123) were PCR amplified. The PPRE containing region of the ADRP promoter was amplified as positive control. <i>Top panel</i>, representative gel scan. <i>Bottom panel</i>, densitometric quantification of three independent experiments in H441 cells. *P<0.01.</p