TolC, EmrB, and MdtB are required for extreme-acid survival.

Abstract

<p>Strains W3110 (K-12 parent strain), JLS1015 (W3110 <i>tolC</i>::<i>kan</i>), JLS1027 (W3110 <i>emrB</i>::<i>kan</i>), and JLS1024 (<i>mdtB</i>::<i>kan</i>) were diluted into LBK pH 2 and exposed with rotation for 2 hours at 37°C after overnight growth to stationary phase in buffered LBK at <b>A</b>) pH 7.0 for non-acid-adapted cells and <b>B</b>) pH 5.5 for acid-adapted cells. Error bars = SEM, n = 6. *Below the level of detection.</p