Two-dimensional gel electrophoretic analysis of pTUC and pTUC-PSO plasmids replicating in Xenopus egg extracts.

Abstract

<p>Plasmids pTUC (A) and pTUC-PSO (B) were incubated 95 min in Xenopus egg extract in the continuous presence of [α-³²P]-dATP. Plasmids were purified, linearized by AflIII digestion and analyzed by 2D gel electrophoresis. Phosphorimager images of the dried gels and interpretative diagrams are shown. (C) Interpretation of the pTUC-PSO specific arcs. (D) Relative intensity of total signal, 1x spot and replication intermediates (RIs) from 3 independent experiments (Exp1, Exp2, Exp3).</p