Specific deletion of <i>Cdc25A</i> in intestinal epithelial cells.

Abstract

<p>(<b>A</b>, <b>B</b>) <i>vR26R</i>;<i>A^f/−</i> and v<i>A^f/−</i> mice were injected with tamoxifen for 5 consecutive days and then sacrificed 3 days after the final injection. Frozen sections of small intestines were prepared and stained with X-gal to visualize Cre-mediated deletion frequencies. Scale bar: 200 µm. (<b>C</b>) Genomic DNA isolated from the small and large intestines of <i>vA^f/−</i> mice 3 days after the final tamoxifen-injection was digested with Bst XI followed by Southern blotting. Deletion frequencies are shown below the each lane. (<b>D</b>) Mice were weighed prior to injection and 3 days after the final tamoxifen injection. Data is presented as mean +/− standard error of the mean (SEM). Asterisks indicate significantly different from WT mice injected with tamoxifen as determined by a Student's t-test. *, P<0.05; **, P<0.01; ***, P<0.001. The actual P-values are 0.86 (<i>vil-Cre-ER^T2</i>), 0.15 (<i>vAKO</i>), 0.87 (<i>vBKO</i>), 0.00007 (<i>vABKO</i>), 0.11(<i>vACKO</i>) and 0.0004 (<i>vTKO</i>).</p