The effects of African green and cynomolgus monkey TRIM5α C-terminal sequences on TRIM5αrh-mediated HIV-1 late restriction activities.

Abstract

<p>(A) Schematic representation of the chimeric TRIM5α constructs between rhesus monkey (Rh, filled), African green monkey (Ag, hatched) and cynomolgus (Cy, dotted) TRIM5α proteins. (B) Western blot analysis of chimeric TRIM5α proteins A/R, R/A and R/C following transfection into 293T cells. Arrow indicates approximate full-length TRIM5α size. (C) Late-restriction activities of chimeric TRIM5α proteins upon co-transfection with pNL4-3 into 293T cells. Viral titers were determined in GHOST(3)R3X4R5 indicator cells and reported as infectious units per ml (IU/ml). Error bars indicate one standard deviation.</p