The “topology screen” assay.

Abstract

<p>(<b>A</b>) Expression constructs. <i>hr3</i> enhancer, baculoviral (BmNPV) homologous region 3 enhancer sequence; pActin, <i>Bombyx mori</i> A3 cytoplasmic actin promoter; MCS, multiple cloning site; actin pA, 3′untranslated region of <i>B. mori</i> actin gene containing polyadenylation signals; Flag, epitope tag; OR; Odorant or opioid receptor ORF; THE, Tobacco etch virus protease recognition site; HR3, <i>Bombyx mori</i> hormone receptor 3. (<b>B</b>) Hypothetical model illustrating possible location of HR3 in both fusion constructs, with respect to the OR orientation (GPCR or not) in the membrane. RORE-bA, response element for retinoic acid receptor-related orphan receptor/basal actin promoter.</p