HIV IIIb makes Huh7 cells sensitive to TRAIL mediated apoptosis.

Abstract

<p>Huh7 cells were pre-incubated or not with AMD3100 for one hour followed by treatment with purified HIV IIIb or mock virus for six hours. Selective points were then treated with skTRAIL for twelve hours. Cells were then fixed, permeabilized, and stained with fluorescently labeled anti-active caspase-3 antibodies and analyzed by flow cytometry. Camptothesin (10 µM) was used as positive control for caspase-3 activation. (A) A single representative dot plot for each group is given. In each plot, the percentage of cells positive for active caspase-3 is shown in the upper right quadrant. (B) Values are expressed as the mean±SD of three independent experiments.</p