Primers and probes.

Abstract

a<p>Fluorescent probe labels are Q670, Quasar 670; CFO560, CAL Fluor Orange 560; FAM, Carboxyfluorescein and CFR610, CAL Fluor Red 610. Quenchers are BHQ, Black Hole Quencher 1 and 2 (Biosearch, Novato, CA).</p>b<p>Position is relative to the coding region of <i>stx</i>1, <i>stx</i>2A or <i>omp</i>A from EDL933.</p>c<p><i>omp</i>A forward and reverse sequences in <b>BOLD</b> are homologous to <i>omp</i>A.</p