qRT-PCR analysis of <i>C4H</i>, <i>CHS</i>, <i>CHI</i> and <i>F3H</i> gene expression in white and red flower petals.

Abstract

<p><i>TEF2</i> was used as an internal control for normalization. qRT-PCR data calculated with the 2^−ΔΔCt method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090842#pone.0090842-Livak1" target="_blank">[48]</a>. The expression level of each gene in red flower petals was arbitrarily set as 1 and its corresponding transcript level in white flower petals was calibrated against red one. Error bars represent standard error for three independent experimental replicates. *, p<0.05, as determined by one-way ANOVA.</p