<i>Pomc</i> enhancers nPE1 and nPE2 function cooperatively.

Abstract

<p>(A) Schematic of nPE mutant <i>Pomc</i> alleles. Green arrowheads show the locations of remnant loxP sites following Cre-mediated excision of the <i>neo</i> gene-targeting selection cassettes (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004935#pgen.1004935.s003" target="_blank">S3 Fig.</a>). (B) <i>Pomc</i> expression measured by qRT-PCR in heads of e10.5 embryos. n = 7–8. (C) Representative <i>Pomc in situ</i> hybridization in sagittal sections of e13.5 embryos. Red box in schematic diagram indicates magnified area. hyp, hypothalamus; pit, pituitary. Scale bar in left panel, 2 mm; right panel, 500 μm. (D-E) Average integrated density of <i>in situ</i> hybridization signal in hypothalamus (D) and pituitary (E). n = 4. Quantitative data are presented as mean + 1 S.E.M. Genotype means were compared by two-tailed t-tests. * <i>P</i> < 0.05, ** <i>P</i> < 0.01, *** <i>P</i> < 0.001 compared to +/+.</p