Organ- and time-specific regulation of genes belonging to the UPP in response to DENV infection.

Abstract

<p>(A) Experimental workflow of transcriptome analysis of <i>Ae</i>. <i>aegypti</i> midgut 8 dpbm. (B) Validation of differentially regulated UPP-specific genes using qRT-PCR. Expression levels of UPP genes in midgut 8 dpbm and head/thorax 21 dpbm were compared. Contrasting expression levels of <i>UBE2A</i>, <i>DDB1</i> and <i>UBE4B</i> were observed, with these genes being down-regulated in the midgut, but up-regulated in the head/thorax when compared with uninfected midguts or heads/thoraces respectively. Mean ± SEM, N = 12. Student’s t test, *p < 0.05. (C) Gene expression levels in individual infected midguts were measured using qRT-PCR, normalized to GAPDH and compared to midguts from uninfected blood fed mosquitoes. Mean ± SEM. N = 12–16. Student’s t test, *p < 0.05.</p