<i>slo-1</i> and <i>slo-2</i> act cell autonomously in promoting the AWC^ON fate.

Abstract

<p>(<b>A</b>, <b>C</b>) AWC phenotypes in wild type, <i>slo-1(eg142lf); slo-2(ok2214lf)</i>, and <i>slo-1(eg142lf); slo-2(ok2214lf)</i> expressing extrachromosomal transgenes <i>odr-3p</i>::<i>slo-1(OE); odr-1p</i>::<i>DsRed</i> (<b>A</b>) or <i>odr-3p</i>::<i>slo-2(OE); odr-1p</i>::<i>DsRed</i> (<b>C</b>). (<b>B</b>) AWC phenotypes of <i>slo-1(eg142lf); slo-2(ok2214lf)</i> mosaic animals containing the extrachromosomal transgene <i>odr-3p</i>::<i>slo-1(OE)</i> in only one AWC cell, inferred by the presence of the coinjection marker <i>odr-1p</i>::<i>DsRed</i> (normally expressed in both AWC). The data was derived from a subset of data in (<b>A</b>). (<b>D</b>) AWC phenotypes of <i>slo-1(eg142lf); slo-2(ok2214lf)</i> mosaic animals containing the extrachromosomal transgene <i>odr-3p</i>::<i>slo-2(OE); odr-1p</i>::<i>DsRed</i> in only one AWC cell. The data was derived from a subset of data in (<b>C</b>). (<b>E</b>) AWC phenotypes in wild-type animals expressing the extrachromosomal transgene <i>nsy-5p</i>::<i>slo-1(T1001Igf); odr-1p</i>::<i>DsRed</i>. <i>slo-1(T1001Igf)</i> contains the <i>ky389gf</i> mutation. (<b>F</b>) AWC phenotypes of mosaic animals containing the extrachromosomal transgene <i>nsy-5p</i>::<i>slo-1(T1001Igf); odr-1p</i>::<i>DsRed</i> in only one AWC cell. The data was derived from a subset of data in (<b>E</b>). AWC^ON was scored as <i>str-2</i>-expressing cell; AWC^OFF was scored as non-<i>str-2</i>-expressing cell. Statistical analysis was performed with a <i>Z</i>-test. Error bars represent the standard error of proportion.</p