(A) Calf thymus histone (50 μg/mL) was pre-mixed with 62.5 μg/mL polysialic acid, 100 U/mL heparin, or 100 nM activated protein C (APC) for 1 h, 10 min, and 30 min, respectively, and then added to hEC for 5 h. Then, U937 cells were added onto histone-treated or untreated hEC layers for 30 min. After washing, the adherent small round U937 cells on hEC layers were counted under a microscope (per ×100 field). (B) The numbers of adherent U937 cells are shown as mean ± SEM of 4 experiments. *P #P < 0.05 versus histone-treated.</p
