Confirmation of gene transfer and inhibition of plasma chemokine activity by M3.

Abstract

Two models of ‘rapid promotion’ or ‘slow progression’ of atherosclerosis were established in which a HFD or regular chow were fed and AdM3 or AdGFP were infused (n = 10–12/group). See “Materials and Methods” for details. Successful gene transfer and expression following adenoviral delivery was determined by Western immunoblotting. A. Culture media from Ad293 cells infected with AdM3 as well as plasma from mice infused with AdGFP and AdM3 were assessed from 4 to 12 weeks post adenoviral delivery following immunoprecipitation with anti-c-myc agarose beads. B. Chemokine activity was measured using the Boyden chamber migration assay. Calcein-AM labelled monocytes were allowed to migrate towards plasma from mice infused with AdGFP or AdM3 (n = 10–12/group). Data are mean±SEM. *pp<0.0001.</p