The struggle to determine susceptibility genes for complex disorders has stimulated
geneticists to develop new approaches. The genetic markers rapidly superseded by
microsatellites since they prolifically throughout the genome and are highly variable in
repeat length and polymorphisms. The Negritos, believed to be the first aborigines
arrived at Peninsular Malaysia, somewhat I 0,000 years ago was thought to be related to
Andaman islanders and Nicobar Islands. They are divided into six groups, namely
Kensiu, Bateq, Mendriq, Jahai, Lanoh and Kintak. In this study, an attempt was made to
optimize the locus D 198220 for GeneS can Analysis purpose. Marker was selected from
Genethon Microsatellite Map (http://www.genlink.wustl.edu/genethon_frame/). DNA
was extracted from the samples collected from three Negrito Tribes in Kelantan: Jahai,
Mendriq and Bateq. A series of PCR optimization were done and run on the ABI 3100
capillary based genetic analyzer. PCR was then amplified on the Mendriq population in
order to examine the PCR condition optimized.
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