Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
The purpose of this study was to detect the presence of perchloric-acid-soluble protein
(PSP) in blast cells of acute leukemic patients and to conduct in-vitro study on the
possibility of blast cells be proliferated into matured cells when treated with PSP. Samples
from acute myeloblastic leukemic (AML) patients and monocytes from healthy donor were
used as a test subject. The small amount of perchloric-acid-soluble 14.5 kDa protein (PSP)
was isolated from monocytes of healthy donor by a combination of trichloroacetic acid
extraction, preparative electrophoresis and CM-Sephadex chromatography. However, this
protein was not found in blast cells of AML patients. The 14.5 kDa protein showed a strong
cross-reactivity when tested with PSP antibody suggesting a close similarity to p14.5 PSP
found in mononuclear phagocytes of human. However in our in-vitro study on the
proliferation of blast cells of AML samples after treated with PSP showed some reaction,
but no significant results. Thus, we believed that the amount of PSP used in this study was
too small which was not enough to play a significant role in cell development and maturity
