In order to establish a successful process of enzymatic hydrolysis of palm oil, for the
production of palmitic acid and oleic acid, the following objectives have been
designated: Screening for potential microorganisms producing a significant amount of
extracellular organic solvent tolerant lipase from indigenous soil samples,
optimization of the physicochemical parameters required to achieve maximum lipase
production in shake flask system, purification of the enzyme using aqueous two phase
system coupled with Sephadex G-75 gel filtration, characterization of the pure
enzyme properties and application of the crude lipase preparation in palm oil
hydrolysis for the production of palmitic and oleic acids
