Chitosan is a natural macromolecular polysaccharide, derived from chitin extracted from
the exoskeleton of some marine invertebrates. This study was designed to evaluate the
expression of P-selectin, thromboxane A2 (TXA2), transforming growth factor-β1 (TGF-
β1) and platelet derived growth factor-AB (PDGF-AB) using enzyme-linked
immunosorbent assays (ELISAs). The chitosan derivatives utilized were 7% N,Ocarboxymethylchitosan
(NO-CMC) (with 0.45 mL collagen), 8% NO-CMC, oligo -
chitosan (O-C) and oligo -chitosan 53 (O-C 53). We found that O-C showed a
significantly superior effect compared to NO-CMC in achieving hemostasis. Post-hoc
multiple comparisons using Scheffe test indicated that p-values between the mean
differences of all the assayed chitosan derivatives were not significant. However, our
analyses successfully identified distinctions between the O-C and NO-CMC test groups
and blood alone. These results may expand the understanding of the ability of chitosan
derivatives to promote inflammatory responses and angiogenesis depending on their
physical and chemical structure
