Ardavanis, Alexandros

Kladi-Skandali, Athina

Scorilas, Andreas

Digital Repository of Hellenic Managing Authority of the Operational Programme &quot;Education and Lifelong Learning&quot; (EDULLL)

International Society for Enzymology   Annual Conference 2015        29 June-1 July, Corfu, Greece    24  BCL2L12: A highly complex gene locus with possible clinical utility in breast cancer Athina Kladi-Skandali1, Alexandros. Ardavanis2, Andreas. Scorilas1 1Department of Biochemistry and Molecular Biology, University of Athens, 2First Department of Medical Oncology, Anticancer Hospital “Saint Savvas”, Athens, Greece Introduction: Apoptosis is a fine tuned and genetically regulated form of cell death, the malfunction of which is among the major hallmarks in malignant transformation of the mammary gland. Among the recently identified members of the BCL2 family of apoptosis-related genes is BCL2L12, which is subjected to alternative splicing, resulting thus in the generation of 13 alternatively spliced variants. Lately, the largest splice variant BCL2L12 v.1 has been studied in various malignancies, revealing its dynamics as a tumor biomarker. The aim of this study was the quantification of BCL2L12 splice variants 1 and 2 (v.1 and v.2) expression at the mRNA level and the assessment of their biomarker potential in breast cancer (BC).  Methods: Total RNA was extracted from 40 pairs of frozen pulverized BC and normal tissues, and was reverse transcribed into first strand cDNA. A sensitive qRT-PCR methodology was developed for the molecular analysis of both BCL2L12 transcript variants BCL2L12 v.1 and v.2, utilising the SYBR Green I chemistry and the comparative CT (2ddCT) method was used for the relative quantification analysis. Finally, the associations of BCL2L12 variants expression with various clinopathological parameters were evaluated by statistical analysis. Results: BCL2L12 v.1 and v.2 mRNA levels transcript variants were found to be significantly (p = 0.003, p = 0.009) higher in malignant compared to their matched non-cancerous breast tissues. Moreover, BCL2L12 v.1 demonstrated increased expression in premenopausal women (p = 0.026) as well as in those with early TNM stage tumors (p = 0.039). Interestingly, significant BCL2L12 v.1 upregulation (p = 0.044) was observed in triple negative BC. Moreover, increased BCL2L12 v.2 expression levels were associated with advanced tumor grade (p = 0.022) and ER-negativity (p = 0.01).  Conclusion: Our preliminary results indicate a possible involvement of BCL2L12 v.1 and v.2 in BC progression and suggest their potential as biomarker in this malignancy.  Acknowledgements: This research has been co-financed by the European Union (European Social Fund – ESF) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: THALES. Investing in knowledge society through the European Social Fund (THALES–UoA–BIOPROMO, MIS 377046).  

BCL2L12: A highly complex gene locus with possible clinical utility in breast cancer

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BCL2L12: A highly complex gene locus with possible clinical utility in breast cancer

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Digital Repository of Hellenic Managing Authority of the Operational Programme "Education and Lifelong Learning" (EDULLL)

BCL2L12: A highly complex gene locus with possible clinical utility in breast cancer

Abstract

Similar works

Full text

Available Versions

Digital Repository of Hellenic Managing Authority of the Operational Programme &quot;Education and Lifelong Learning&quot; (EDULLL)

Digital Repository of Hellenic Managing Authority of the Operational Programme "Education and Lifelong Learning" (EDULLL)