AF Haag

B Khor

Charlotte E. Nicholl

Craig Pattinson

David A. Stead

E Molitoris

Emad M. El-Omar

EN Ilina

Georgina L. Hold

GL Hold

H Sakon

H Sokol

HL Greetham

HM Wexler

I Mangin

I Mukhopadhya

Indrani Mukhopadhya

J Engberg

JG Fox

JM Thomson

John M. Thomson

L Zhang

LB Reller

LD Kalischuk

M Alispahic

MB Skirrow

MF Andreasen

MS Silverberg

MV Selma

P Lasch

P Marteau

R Hansen

R Kolinska

Richard Hansen

Richard K. Russell

S Kumar

S Rozen

S Schippa

Stefan Bereswill

Susan H. Berry

Yazeid A. Alhaidan

PLoS ONE

English

PubMed

Crossref

A Comprehensive Evaluation of Colonic Mucosal Isolates of Sutterella wadsworthensis from Inflammatory Bowel Disease

Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls

Mukhopadhya, I.

Hansen, R.

Nicholl, C.E.

Alhaidan, Y.A.

Thomson, J.M.

Berry, S.H.

Pattinson, C.

Stead, D.A.

Russell, R.K.

El-Omar, E.M.

Hold, G.L.

Enlighten: Publications

A comprehensive evaluation of colonic mucosal isolates of sutterella wadsworthensis from inflammatory bowel disease

Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls. © 2011 Mukhopadhya et al

Mukhopadhya, I

Hansen, R

Nicholl, CE

Alhaidan, YA

Thomson, JM

Berry, SH

Pattinson, C

Stead, DA

Russell, RK

El-Omar, EM 

Hold, GL 

UNSWorks

Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls.</p

Mukhopadhya, Indrani

Hansen, Richard

Nicholl, Charlotte E.

Alhaidan, Yazeid A.

Thomson, John M.

Berry, Susan H.

Pattinson, Craig

Stead, David A.

Russell, Richard K.

El-Omar, Emad M.

Hold, Georgina L.

University of Dundee Online Publications

A comprehensive evaluation of colonic mucosal isolates of <i>sutterella wadsworthensis</i> from inflammatory bowel disease

Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls

Charlotte E Nicholl

Yazeid A Alhaidan

John M Thomson

Susan H Berry

David A Stead

Richard K Russell

Emad M El-Omar

Georgina L Hold

Directory of Open Access Journals

A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease.

Discovery Research Portal

Mukhopadhya Indrani

Hansen Richard

Nicholl Charlotte E.

Alhaidan Yazeid A.

Thomson John M.

Berry Susan H.

Pattinson Craig

Stead David A.

Russell Richard K.

El-Omar Emad M.

Hold Georgina L.

Public Library of Science (PLOS)

 from Inflammatory Bowel Disease

Nicholl, Charlotte E

Alhaidan, Yazeid A

Thomson, John M

Berry, Susan H

Stead, David A

Russell, Richard K

El-Omar, Emad M

Hold, Georgina L

Aberdeen University Research

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Campylobacter enteritis: a ‘‘new’’ disease.

Controlled evaluation of an improved selective medium for isolation of Campylobacter jejuni from human feces [abstract] 83rd Annu Meet Am Soc Microbiol: C274:

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Primer3 on the WWW for general users and for biologist programmers.

Sources and antimicrobial susceptibilities of Campylobacter gracilis and Sutterella wadsworthensis. Clin Infect Dis 25(Suppl 2):

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Specificities of the fecal microbiota in inflammatory bowel disease.

Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces.

Sutterella stercoricanis sp. nov., isolated from canine faeces.

Sutterella wadsworthensis gen. nov., sp. nov., bile-resistant microaerophilic Campylobacter gracilis-like clinical isolates.

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Toward an integrated clinical, molecular and serological classification of inflammatory bowel disease: Report of a Working Party of the 2005 Montreal World Congress of Gastroenterology.

A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease

Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis.

Biochemical characterization of Sinorhizobium meliloti mutants reveals gene products involved in the biosynthesis of the unusual lipid A very long-chain fatty acid.

Comparative genotypic and pathogenic examination of Campylobacter concisus isolates from diarrheic and nondiarrheic humans.

Direct bacterial profiling by matrix-assisted laser desorptionionization time-of-flight mass spectrometry for identification of pathogenic Neisseria.

Dominant genotypes in mucosa-associated Escherichia coli strains from pediatric patients with inflammatory bowel disease.

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Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.

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A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease

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