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基因工程大肠杆菌合成γ-聚谷氨酸
Authors
刘会洲
李强
+3 more
王丹
邢建民
马婕
Publication date
1 January 2009
Publisher
Abstract
利用PCR方法,从自身不合成γ-聚谷氨酸(γ-PGA)的Bacillussubtilis168菌的基因组DNA中扩增出γ-PGA合成基因ywsC,ywtA和ywtB,测序并对该基因编码区进行序列分析,比对结果表明,扩增的ywsC,ywtA和ywtB与文献报道的相似度为100%.将3个基因连接到pTrcHisA载体后转化至E.coliTOP10及E.coliBL21(DE3)宿主菌表达,结果宿主菌细胞均具备了γ-PGA生物合成能力,产量最高达到0.134g/L
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/46158
Last time updated on 11/04/2021
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/10245
Last time updated on 09/11/2016
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/32481
Last time updated on 05/12/2019