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超大孔聚苯乙烯微球固定蛋白质
Authors
孔英俊
曲建波
+5 more
曲瑞芳
罗坚
苏志国
马光辉
马润宇
Publication date
1 January 2009
Publisher
Abstract
采用琼脂糖对孔径为300~500nm的超大孔聚苯乙烯(MPS)微球进行亲水化修饰,并在修饰后的表面上偶联病毒蛋白颗粒(RV),用于蛋白质的分离纯化实验.结果表明,经过修饰的MPS层析介质具有优异的流体力学性能,层析过程的最高流速可达1120cm/h,对病毒蛋白颗粒的固定量为0.638mg/g,是商品化介质Sepharose 4FF的2倍.采用RV-MPS介质对病毒蛋白颗粒抗体进行了分离纯化,收率为29.22%,高于Sepharose 4FF的18.92%
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/46736
Last time updated on 11/04/2021
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/10303
Last time updated on 09/11/2016
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/31525
Last time updated on 05/12/2019