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反相色谱法研究人粒细胞集落刺激因子的离子交换色谱复性和稀释复性
Authors
丁虹
李京京
罗天乐
苏志国
Publication date
1 January 2005
Publisher
Abstract
以反相色谱分析为主要手段,结合活性测定,研究了以包含体形式表达的重组人粒细胞集落刺激因子(rhG-CSF)的稀释复性和离子交换色谱复性. 建立了L-精氨酸离子交换复性蛋白质的方法,将变性、还原的rhG-CSF吸附到高浓度变性剂平衡的离子交换色谱柱上,用L-精氨酸作洗脱剂进行梯度洗脱并同时脱除变性剂,实现了rhG-CSF的复性. 与稀释复性相比,色谱复性的rhG-CSF处于一种中间状态,呈现快速而不同步的动力学特点,这与色谱复性的梯度洗脱及固定相的吸附有关. 同时发现了对复性峰进一步稀释并且温育则可以提高其活性的新现象
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/4735
Last time updated on 09/11/2016
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/35948
Last time updated on 05/12/2019
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
See this paper in CORE
Go to the repository landing page
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oai:ir.ipe.ac.cn:122111/44110
Last time updated on 11/04/2021