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PEG沉淀结合层析分离重组乙肝病毒表面抗原
Authors
周卫斌
孙娟
+5 more
张焱
李岩
苏志国
董爱华
闭静秀
Publication date
1 January 2006
Publisher
Abstract
应用聚乙二醇(PEG)沉淀结合层析技术纯化乙肝病毒表面抗原(HBsAg),对PEG浓度、pH值、离子强度等影响PEG沉淀的因素进行了正交实验研究.结果表明,浓度为0.12g/mL的PEG6000在4℃,pH9.0条件下沉淀HBsAg纯化效果比较理想,纯化倍数达3.7,回收率96.8%,有效地去除了生物大分子杂质和牛血清白蛋白(BSA),PEG在后续的凝胶过滤中可以除去,整个工艺的回收率可提高到41%
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/46655
Last time updated on 11/04/2021
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/4552
Last time updated on 09/11/2016
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/31634
Last time updated on 05/12/2019