番红花球茎的快速高频诱导

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Publication date
1 January 2007
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Abstract

以体外培养的脱毒番红花芽为材料,考察了外源激素及活性炭对球茎诱导的作用.外源激素水平对球茎诱导有重要影响,较低的激素浓度下球茎诱导时间较短,较高的激素浓度下诱导出的球茎较大,但诱导时间延长.培养基中添加活性炭后,由于活性炭的吸附作用降低了游离激素水平,显著缩短了球茎诱导时间,后期激素的缓慢释放提高了球茎质量.在优化的1/2MS+5mg/LNAA+5mg/L6-BA+0.5g/LAC培养基中,5周球茎诱导率高达85.7%,球茎均重0.53g,满足移栽要求

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