Clark, Andrew

Lincoln, Per

McCauley, Micah J.

Paramanathan, Thayaparan

Rouzina, Ioulia

Westerlund, Fredrik

Williams, Mark C.

Virtual Commons - Bridgewater State University

Abstract Submittedfor the MAR15 Meeting ofThe American Physical SocietyThreading moieties play a signicant role in determining theDNA binding properties of binuclear ruthenium complexes THAYAPARANPARAMANATHAN, Department of Physics, Bridgewater State University, Bridge-water, MA, ANDREW CLARK, Department of Physics, Northeastern University,Boston, MA, FREDRIK WESTERLUND, PER LINCOLN, Department of Chem-ical and Biological Engineering, Chalmers University of Technology, Gothenburg,MICAH J. MCCAULEY, Department of Physics, Northeastern University, Boston,MA, IOULIA ROUZINA, Department of Biochemistry, Molecular Biology and Bio-physics, University of Minnesota, Minneapolis, MN, MARK C. WILLIAMS, De-partment of Physics, Northeastern University, Boston, MA | Binuclear rutheniumcomplexes are of interest due to their selective DNA binding properties, which makethem potential candidates for chemotherapy. These dumbbell shaped molecules haveto thread through the DNA base pairs to reach their nal threaded intercalationstate. Here we study the binuclear ruthenium complex, -[-bidppz(bpy)4Ru2]4+and compare it with the previously studied -[-bidppz(phen)4Ru2]4+. Both havethe same intercalating bridge unit, but dierent threading moieties. In this study,we stretch a single DNA molecule held with optical tweezers in the presence of theligand at various concentrations and hold the DNA at constant force until an equilib-rium DNA elongation is reached. The extension of the DNA obtained as a functionof time during binding yields the kinetics and equilibrium binding properties ofthe ligand. The preliminary data suggests that the binuclear complex with bpy inthe threading moiety shows stronger anity and an order of magnitude faster onrate, compared to its counterpart with phen in the threading moiety. This conrmsthe hypothesis that the extra aromatic ring of phen interferes with the threadingintercalation process.Thayaparan ParamanathanDepartment of Physics, Bridgewater State University, Bridgewater, MADate submitted: 14 Nov 2014 Electronic form version 1.4

Threading Moieties Play a Significant Role in Determining the DNA Binding Properties of Binuclear Ruthenium Complexes

http://absimage.aps.org/image/MAR15/MWS_MAR15-2014-008547.pdf

Threading Moieties Play a Significant Role in Determining the DNA Binding Properties of Binuclear Ruthenium Complexes

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