Multiplexed spatial profiling of Hodgkin Reed-Sternberg cell neighborhoods in Classic Hodgkin lymphoma unveils distinct immune escape mechanisms

Abstract

<p>***Please refer to Version v3***</p> <p> </p> <p>Raw TIFF images and corresponding single-cell data from multiplexed imaging (Leica Cell DIVE) of 29 proteins in 587 fields of view across 30 classic Hodgkin lymphoma patients.</p> <p>Bulk RNA sequencing (NanoString IO360 panel) raw and normalized data for 32 classic Hodgkin lymphoma patients.</p> <p>Meta data containing information about patients, cell type and state information, and statistical comparisons.</p><p>Individual file descriptions</p> <ul> <li>RawData.csv <ul> <li>Raw NanoString data (32 patients)</li> </ul> </li> <li>NormaizedData.csv <ul> <li>Normalized NanoString data (32 patients)</li> </ul> </li> <li>Files beginning with HL_* <ul> <li>Normallized single-cell mpIF data (30 patients, 1 per file)</li> </ul> </li> <li>HodgkinLymphoma_Samples.xlsx <ul> <li>Meta data containing patient-level information</li> </ul> </li> <li>HodgkinLymphoma_FOVs.xlsx <ul> <li>Meta data containing field of view-level information</li> </ul> </li> <li>HodgkinLymphoma_Markers.xlsx <ul> <li>Meta data containing information on markers in cell dive panel</li> </ul> </li> <li>HodgkinLymphoma_CellTypes.xlsx <ul> <li>Meta data containing cell type definitions</li> </ul> </li> <li>HodgkinLymphoma_CellStates.xlsx <ul> <li>Meta data containing cell state information</li> </ul> </li> <li>HodgkinLymphoma_Questions.xlsx <ul> <li>Meta data containing list of statistical comparisons</li> </ul> </li> </ul> <p>*Note: all meta data is formatted for use with the pipeline</p&gt