ObjectiveSeeking a comprehensive therapeutic approach to address multiple pathological processes in Diabetic Cardiomyopathy (DCM).MethodsRandomly divided all mice into control group, DCM group, DCM+PFTJ group, DCM+HIIT group, and DCM+PFTJ+HIIT group. Used intraperitoneal injection of streptozotocin combined with high-fat diet to treat mice, and treated them according to the experimental plan for 16 weeks. Recorded the heart weight to body weight ratio (HW/BW), detected cardiac function using small animal ultrasound imaging system, and assessed the pathological damage of the heart by detecting the levels of lactate dehydrogenase (LDH), creatine kinase (CK), troponin I, IL-6, IL-1β, and TNF-α in the serum, combined with hematoxylin and eosin staining. At the same time, detected the expression of NLRP3 and Cleaved caspase-1 proteins in the myocardial tissue.ResultsCompared with the DCM group, the degree of myocardial fiber disarray and myocardial fibrosis in mice of the DCM+PFTJ group and DCM+HIIT group was significantly reduced, with the DCM+PFTJ+HIIT group showing the best effected in improving myocardial structure. Cardiac function indicators (left ventricular ejection fraction and fractional shortening) were improved in the DCM+PFTJ group and DCM+HIIT group, with the DCM+PFTJ+HIIT group showing a more significant effect; the HW/BW of mice in the DCM+PFTJ+HIIT group increased, and the levels of LDH, CK, troponin I, IL-6, IL-1β, and TNF-α in the serum were significantly decreased. In their myocardial tissue, the expression levels of NLRP3 and Cleaved caspase-1 proteins were also significantly reduced.ConclusionThe combination of PFTJ and HIIT significantly improves the cardiac condition of DCM mice by improving cardiac function and myocardial damage, and its possible mechanism may be related to the inhibition of the NLRP3 inflammasome
