Effects of N1-methylated purines on DNA double helical structures and stabilities.

Abstract

Zhan, Yingqian.Thesis (M.Phil.)--Chinese University of Hong Kong, 2007.Includes bibliographical references (leaves 49-54).Abstracts in English and Chinese.Title Page --- p.iThesis Committee --- p.iiAbstract (English version) --- p.ivAbstract (Chinese version) --- p.vAcknowledgment --- p.viTable of Contents --- p.viiList of Tables --- p.ixList of Figures --- p.xList of Abbreviations and Symbols --- p.xiiiChapter 1. --- Introduction --- p.1Chapter 1.1 --- DNA Methylation --- p.1Chapter 1.2 --- DNA Structure --- p.2Chapter 1.2.1 --- Nomenclature Scheme for DNA --- p.2Chapter 1.2.2 --- Conformations of DNA --- p.5Chapter 1.2.3 --- Base Pair Scheme --- p.5Chapter 1.2.4 --- Sugar Conformation --- p.7Chapter 1.2.5 --- Backbone Conformation --- p.7Chapter 1.3 --- DNA Melting --- p.7Chapter 1.4 --- Objective of this Work --- p.8Chapter 2. --- Materials and Methodology --- p.9Chapter 2.1 --- Sequence Design --- p.9Chapter 2.2 --- Sample Preparation --- p.10Chapter 2.3 --- Resonance Assignment --- p.10Chapter 2.3.1 --- Proton Resonance Assignment --- p.11Chapter 2.3.2 --- Phosphorous Resonance Assignment --- p.14Chapter 2.4 --- Determination of Sugar Conformation --- p.15Chapter 2.5 --- Determination of Backbone Conformation --- p.16Chapter 2.6 --- Thermodynamic study on DNA --- p.18Chapter 3. --- Effects of N1-methylated Adenine on DNA Structure and Stability --- p.21Chapter 3.1 --- Overview --- p.21Chapter 3.2 --- Resonance Assignment Results --- p.21Chapter 3.2.1 --- 1H Resonance Assignments --- p.21Chapter 3.2.2 --- 31P Resonance Assignments --- p.23Chapter 3.3 --- Base Pair Structures --- p.24Chapter 3.4 --- Sugar Conformation --- p.26Chapter 3.5 --- Backbone Conformation --- p.27Chapter 3.6 --- Thermodynamic Stability Study --- p.29Chapter 4. --- Effects of N1-methylated Guanine on DNA Structure and Stability --- p.31Chapter 4.1 --- Overview --- p.31Chapter 4.2 --- Resonance Assignment Results --- p.31Chapter 4.2.1 --- 1H Resonance Assignments --- p.31Chapter 4.2.2 --- 31P Resonance Assignments --- p.33Chapter 4.3 --- Base Pair Structures --- p.34Chapter 4.4 --- Sugar Conformation --- p.36Chapter 4.5 --- Backbone Conformation --- p.39Chapter 4.6 --- Thermodynamic Stability Study --- p.41Chapter 5. --- Conclusion and Future work --- p.43Appendix I H1'-H6/H8 region of NOESY spectra of TmeA at 25 °C with different mixing times --- p.44"Appendix II 3JH1'h2"", 3JH1'h2"", and %S of TmeA and refTA" --- p.45"Appendix III 3JH1' h2'，3JH1'-H2"", and %S of CmeG and refCG" --- p.46"Appendix IV 31P chemical shifts, 3JH3'p and %B1 of TmeA, and refTA" --- p.47"Appendix V 31P chemical shifts, 3JH3'P and %B1of CmeG, and refCG" --- p.48References --- p.4